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Similar limitations had been found when 5 of seven flavourings were found to trigger cytotoxicity in human bronchial epithelial cells [87]. Results from in vitro research are in general settlement with the limited number of in vivo research. For instance, in an analysis utilizing major human umbilical vein endothelial cells (HUVEC) exposed to eleven commercially-available vapours, 5 have been discovered to be acutely cytotoxic, and solely three of these contained nicotine [24].